In order to enhance scaffold bioactivity for human mesenchymal stem cells (hMSCs) culture, scaffold surface modification through the physical absorption of collagen I and fibronectin (FN) were investigated by cell live/dead staining and cell viability assays. Herein, 3D printing-based strategy via the cryo-printing technology was employed to fabricate customized polyurethane (PU) porous scaffolds that mimic native meniscus.

3D printing of polymer scaffolds holds the promise of precisely constructing complex tissue architecture, however the resultant scaffolds usually lack of sufficient bioactivity to effectively generate new tissue. The poor regenerative capability and structural complexity make the reconstruction of meniscus particularly challenging in clinic. # indicates P < 0.05 for difference between pre and all hydrated groups. Significance is denoted as * P < 0.01, and indicates significance over all other bars within the graph. Fiber diameter is significantly greater at all hydrated time points than fibers before hydration (pre), but no significant difference was found among hydration time points. Graph depicts percent swelling of fibers, which remains consistent at about 50% swelling over 48 hours, though fibrous morphology is maintai- ned. (D) RBM nanofibers resist morphology changes with hydration. All parameter sets which include 1% (w/v) concen- tration are included in that bar, regardless of the other parameters. Parametric manipulations, especially with flow rate (B) and concentration (C) exert changes in fiber diameter. (A) Representative scanning electron micrograph indicating successful fiber formation at 3% (w/v) initial concentration, 1.5mL/hr flow rate. Parametric manipulations exert control over RBM fiber diameter.

Fityk rbm diameter